Lab tests
Vitamin D, 25-hydroxy cost in Jacksonville, FL
Vitamin D, 25-hydroxy is a lab test. Many labs sell tests directly to the public for a flat cash price, often far below hospital prices.
Typical cash price
In Florida
From 230 hospital and 17 clinic prices in Florida.
Nationwide typical: $101 (most listed prices $25.20–$335).
Usually included: the facility fee.
Good to know: 1,25-dihydroxy (82652) is a different test.
Cheapest in Jacksonville, FL
Total price
Vitamin D, 25-hydroxy
The facility fee
How totals are compared
Every total covers the same bundle: the facility fee. Where a hospital does not publish a part, the typical published amount is added and the total is marked estimated.
Near Jacksonville, FL, from 14 providers. Most listed prices: $5.55–$297.
Cheapest totals nearby
- Ascension St. Vincent's Clay County (St. Vincent's Medical Center, Inc.)$5.55Hospital · Middleburg · ~20 miAll parts published by the hospital
- Ascension St. Vincent's Riverside (St. Vincent's Medical Center, Inc.)$5.55Hospital · Jacksonville · nearbyAll parts published by the hospital
- Ascension St. Vincent's Southside (St. Luke's-St. Vincent's HealthCare, Inc.)$5.55Hospital · Jacksonville · 7.3 miAll parts published by the hospital
- Ascension St. Vincent's Clay County (St. Vincent's Medical Center, Inc.)Vitamin D testFacility fee only$5.55est. $5.55 total
Doctor fees are billed separately.
Source: Ascension St. Vincent's Clay County (St. Vincent's price fil · checked Oct 2026 - Ascension St. Vincent's Riverside (St. Vincent's Medical Center, Inc.)Vitamin D testFacility fee only$5.55est. $5.55 total
Doctor fees are billed separately.
Source: Ascension St. Vincent's Riverside (St. Vincent's M price fil · checked Oct 2026 - Ascension St. Vincent's Southside (St. Luke's-St. Vincent's HealthCare, Inc.)Vitamin D testFacility fee only$5.55est. $5.55 total
Doctor fees are billed separately. Location not independently confirmed.
Source: Ascension St. Vincent's Southside (St. Luke's-St. price fil · checked Oct 2026 - Ascension St. Vincent's St. Johns County (St. Vincent's Health System, Inc.)Vitamin D testFacility fee only$5.55est. $5.55 total
Doctor fees are billed separately.
Source: Ascension St. Vincent's St. Johns County (St. Vinc price fil · checked Oct 2026 - $6.60est. $6.60 total
Doctor fees are billed separately.
Source: Shands Jacksonville Medical Center price file · checked Oct 2026 - $12est. $12 total
Doctor fees are billed separately.
Source: HCA Florida Memorial Hospital price file · checked Oct 2026 - $14.26est. $14.26 total
Doctor fees are billed separately.
Source: HCA Florida Orange Park Hospital price file · checked Oct 2026 - $45est. $45 total
Doctor fees are billed separately. Location not independently confirmed.
Source: Brooks Rehabilitation Hospital – University Campus price fil · checked Oct 2026 - $58est. $58 totalSource: chasevillelabs.com · checked Oct 2026
- $69est. $69 total
Doctor fees are billed separately.
Source: Encompass Health Rehabilitation Hospital of Jackso price fil · checked Oct 2026 - $69.56est. $69.56 total
Doctor fees are billed separately.
Source: Reunion Rehabilitation Hospital Jacksonville price file · checked Oct 2026 - $238est. $238 total
Doctor fees are billed separately.
Source: Mayo Clinic Florida price file · checked Oct 2026
Common questions
Do I need a doctor's order?
In most states, direct-to-consumer labs can sell many tests without a separate doctor visit. Some states and some tests still require an order.
Is the blood draw included?
Usually yes for lab stores and booking sites. Hospitals sometimes bill the draw (venipuncture) separately.
Why are prices so different?
Each hospital and clinic sets its own cash price. Hospitals publish prices because federal rules require it; clinics and booking sites set prices to compete. The same service can cost several times more at one place than at another nearby.
Can I pay cash if I have insurance?
Yes. You can choose to self-pay. A cash price is often lower than what you would pay before meeting your deductible. Cash payments may not count toward your deductible, so check with your plan. Our cash vs. insurance tool can help you decide.
What is a Good Faith Estimate?
If you are uninsured or paying cash, providers must give you a Good Faith Estimate of the cost on request. If your final bill is $400 or more above the estimate, you can dispute it.